물리화학 실험실
물리화학 실험실
 학과소개 연구실/실험실 물리화학 실험실
담당교수 이동국 교수
연구분야 - 항균 펩타이드(antimicrobial peptides)의 구조 및 작용 기작 연구
- 제2형 당뇨병 관련 hIAPP 펩타이드의 구조 및 지질막과 상호 작용연구
- 고분자 물질(polymers)의 구조 및 분자 운동 연구
- 액체 결정물질(liqulid crystals)의 구조와 기능 상관 관계 연구
- 모델 세포막에서 생체 분자(biomolecules)들의 구조 연구를 위한 고체 핵자기 공명 실험 방법 개발
분야설명 상기의 각 분야의 연구에 solid-state Nuclear Magnetic Resonance Spectrometer, Differential Scanning Calorimeter, FTIR. UV-VIS, 그리고 Fluorometer를 이용하여 펩타이드를 포함한 생체시료 및 고분자 물질들의 구조 분석, 물성 연구, 그리고 이들 연구를 위한 새로운 실험 방법 개발에 관한 연구를 수행하고 있다
논문 및 저서
  • - Yufeng Wei, Dong-Kuk Lee, Kevin J. Hallock and A. Ramamoorthy, Chem. Phys. Lett., 351 (2002) 42-46, “One-Dimensional 1H-Detected Solid-State NMR Experiment to Determine Amide-1H Chemical Shifts in Peptides.”
  • - Katherine A. Henzler, Dong-Kuk. Lee, and A. Ramamoorthy, Biopolymers (2002), 64, 246-254, “Effect of sample preparation on the poly-L-alanine secondary structure in the solid state: Insight into a-helix and b-sheet interconversion and stability.”
  • - Kevin J. Hallock, Katherine A. Henzler, Dong-Kuk Lee and A. Ramamoorthy, Biophys. J, (2002), 82, 2499-2503, “An Innovative Procedure Using A Sublimable Solid To Align Lipid Bilayers for Solid-State NMR Studies.”
  • - Kevin J. Hallock, Dong-Kuk Lee ,John Omnaas, Henry I. Mosberg, and A. Ramamoorthy, Biophys. J, (2002), 83, 1004-1013, “Membrane Composition Determines Pardaxin’s Mechanism of Lipid Bilayer Disruption.”
  • - Srinivasan C. Shekar, Dong-Kuk Lee, and A. Ramamoorthy, J. Magn. Reson., (2002), 157, 223-234. “Chemical shift anisotropy and offset effects in cross polarization solid-state NMR spectroscopy.”
  • - Yufeng Wei, Dong-Kuk Lee, and A. Ramamoorthy, J. Magn. Reson., (2002), 158, 23-35. “A 2D MAS Solid-State NMR Method to Recover the Amplified Heteronuclear Dipolar and Chemical Shift Anisotropic Interactions.”
  • - J. S. Santos, Dong-Kuk Lee, K. J. Hallock and A. Ramamoorthy, Recent Research Developments in Physical Chemistry, Transworld Research Network, 6(2002), 179-211, ISBN 81-7895-042-1, 37/661(2), Fort P.O., Trivandrum-695 023, Kerala, India “Cholesterol and antidepressant drug interaction with phospolipid: A solid state NMR Study.”
  • - Kevin J. Hallock, Dong-Kuk Lee, and A. Ramamoorthy, Biophys. J, (2003), 84, 3052-3060, “MSI-78, an Analogue of the Magainin Antimicrobial Peptides, Disrupts Lipid Bilayer Structure via Positive Curvature Strain”
  • - Srikanth S. Kidambi, Dong-Kuk Lee, and A. Ramamoorthy, Inorganic Chem., (2003), 42, 3142-3151, “Interaction of Cd and Zn with Biologically Improtant Ligands Characterized Using Solid-State NMR and ab initio Calculations”
  • - Katherine A. Henzler Wildman, Dong-Kuk. Lee, and A. Ramamoorthy, (2003), 42, 6545-6558, Biochemistry. “Mechanism of Lipid Bilayer Disruption by the Human Antimicrobial Peptide, LL37”.
  • - Katherine A. Henzler, Erin E. Wilson, Dong-Kuk. Lee, and A. Ramamoorthy, (2003), Solid-state NMR. 24,94-109. “Determination of the Conformation and
  • - Kevin J. Hallock, Dong-Kuk Lee, and A. Ramamoorthy, Biophys. J, (2003), 84, 3052-3060, “MSI-78, an Analogue of the Magainin Antimicrobial Peptides, Disrupts Lipid Bilayer Structure via Positive Curvature Strain”
  • - Srikanth S. Kidambi, Dong-Kuk Lee, and A. Ramamoorthy, Inorganic Chem., (2003), 42, 3142-3151, “Interaction of Cd and Zn with Biologically Improtant Ligands Characterized Using Solid-State NMR and ab initio Calculations”
  • - Katherine A. Henzler Wildman, Dong-Kuk. Lee, and A. Ramamoorthy, (2003), 42, 6545-6558, Biochemistry. “Mechanism of Lipid Bilayer Disruption by the Human Antimicrobial Peptide, LL37”
  • - Katherine A. Henzler, Erin E. Wilson, Dong-Kuk. Lee, and A. Ramamoorthy, (2003), Solid-state NMR. 24,94-109. “Determination of the Conformation and Stability of Simple Homopolypeptides Using Solid-State NMR”
  • - J. S. Santos, Dong-Kuk Lee, and A. Ramamoorthy, Mag. Reson. in Chem (2004) 42, 105-114, “Effects of Anti-Depressants on the Conformation of Phospholipid Headgroup studied by Solid-State NMR technique.”
  • - Dong-Kuk. Lee, Katherine A. Henzler Wildman, and A. Ramamoorthy, JACS.(2004) 126, 2318-2319, “Solid-State NMR Spectroscopy of Aligned Lipid Bilayers at Low Temperatures.”
  • - Almut Mecke, S. Uppuluri, T. J. Sassanella, D.-K. Lee, A. Ramamoorthy, J. R. Baker, Jr., B. G. Orr, M. M. Banaszak Holl, Chemistry and Physics of Lipids (2004) 132, 3-14, “Direct Observation of Lipid Bilayer Distruption by Polyamidoamine Dendrimers.”
  • - Dong-Kuk. Lee, T. Narasimhaswamy and A. Ramamoorthy, Chem. Phys. Lett. (2004), 399, 359-362, “PITANSEMA, A Low-Power PISEMA Solid-State NMR Experiment.”
  • - Caroline D. Pointer-Keenana, Dong-Kuk Lee, Kevin Hallock, Anmin Tan, Robert Zand, Ayyalusamy Ramamoorthy, Chem. Phys. Lipids (2004), 132, 47-54, “Investigation of the interaction of myelin basic protein with phospholipid bilayers using solid-state NMR spectroscopy.”
  • - Fernando Porcellil, Bethany Buck, Dong-Kuk Lee, Kevin J. Hallock, AyyalusamyRamamoorthy, and Gianluigi Veglia, J. Biolo. Chem.,“ (2004), 279(44),45815-45823, Structure and Orientation of Pardaxin Determined by NMR Experiments in Model Membranes.”
  • - J. S. Santos, Dong-Kuk Lee, and A. Ramamoorthy, Mag. Reson. in Chem (2004) 42, 105-114, “Effects of Anti-Depressants on the Conformation of Phospholipid Headgroup studied by Solid-State NMR technique.”
  • - Dong-Kuk. Lee, Katherine A. Henzler Wildman, and A. Ramamoorthy, JACS.(2004) 126, 2318-2319, “Solid-State NMR Spectroscopy of Aligned Lipid Bilayers at Low Temperatures.”
  • - Almut Mecke, S. Uppuluri, T. J. Sassanella, D.-K. Lee, A. Ramamoorthy, J. R. Baker, Jr., B. G. Orr, M. M. Banaszak Holl, Chemistry and Physics of Lipids (2004) 132, 3-14, “Direct Observation of Lipid Bilayer Distruption by Polyamidoamine Dendrimers.”
  • - Dong-Kuk. Lee, T. Narasimhaswamy and A. Ramamoorthy, Chem. Phys. Lett. (2004), 399, 359-362, “PITANSEMA, A Low-Power PISEMA Solid-State NMR Experiment.”
  • - Caroline D. Pointer-Keenana, Dong-Kuk Lee, Kevin Hallock, Anmin Tan, Robert Zand, Ayyalusamy Ramamoorthy, Chem. Phys. Lipids (2004), 132, 47-54, “Investigation of the interaction of myelin basic protein with phospholipid bilayers using solid-state NMR spectroscopy.”
  • - Fernando Porcellil, Bethany Buck, Dong-Kuk Lee, Kevin J. Hallock, AyyalusamyRamamoorthy, and Gianluigi Veglia, J. Biolo. Chem. (2004), 279(44),45815-45823, Structure and Orientation of Pardaxin Determined by NMR Experiments in Model Membranes.”
  • - Almut Mecke, Dong-Kuk Lee, A. Ramamoorthy, B. G. Orr, M. M. Banaszak Holl, Biophys J. (2005) 89, 4043-4050, “Membrane thinning due to antimicrobial peptide binding: An atomic force microscopy study of MSI-78 in lipid bialyers.”
  • - Sathiah Thennarasu, Dong-Kuk Lee, A. Poon, K. E. Kawulka, J. C. Vederas, Ayyalusamy Ramamoorthy, Chem. Phys. Lipids 137 (2005) 38?51, “Membrane permeabilization, orientation, and antimicrobial mechanism of subtilosin A.” Published on web 07/20/05
  • - Almut Mecke, Dong-Kuk Lee, A. Ramamoorthy, B. G. Orr, M. M. Banaszak Holl, Langmuir. (2005) 21, 8588-8590, “Synthetic and natural polycationic polymer nanopolymers interact selectively with fluid-phase domains of DMPC lipid bilayers.” Published on web 08/10/05
  • - T. Narasimhaswamy, M. Monette, D. K. Lee and A. Ramamoorthy, J. Phys. Chem, B. (2005) 109, 19696-19703,” Solid-State NMR Characterization and Determination of the Orientational Order of a Nematogen.”
  • - T. Narasimhaswamy, D. K. Lee, N. Somanathan and A. Ramamoorthy, Chem. Mater. (2005), 17, 4567-4569,” Solid-State NMR Characterization of a Novel Thiophene-Based Three Phenyl Ring Mesogen”
  • - K. Yamamoto, V. L. Ermakov, Dong-Kuk. Lee, and A. Ramamoorthy, Chem. Phys. Lett. (2005), 408, 118-122 “PITANSEMA-MAS, a Solid-State NMR method to measure heteronuclear dipolar couplings under MAS.” Available online 27 April 2005
  • - Ayyalusamy Ramamoorthy, Sathiah Thennarasu, Anmin Tan, Dong-Kuk Lee, Carol Clayberger and Alan M. Krensky, BBA, Biomembrane, (2006), 1758, 154-163, “Cell selectivity correlates with membrane-specific interactions: A case study on the antimicrobial peptide G15 derived from granulysin.” Available online 10 March 2006.
  • - Dong-Kuk Lee, Byung Soo Kwon, and Ayyalusamy Ramamoorthy, “Freezing Point Depression of Water in Phospholipid Membranes: A Solid-State NMR Study”, Langmuir, (2008), 13598 ? 13604.
  • - Ramamoorthy A, Lee DK, Santos JS, Henzler-Wildman KA. Nitrogen-14 Solid-State NMR Spectroscopy of Aligned Phospholipid Bilayers to Probe Peptide-Lipid Interaction and Oligomerization of Membrane Associated Peptides. J. Am. Chem. Soc. 130, (2008), 11023-11029.
  • - Jeffrey Barry, Michelle Fritz, Jeffrey R. Brender, Pieter E. S. Smith, Dong-Kuk Lee and Ayyalusamy Ramamoorthy, J. Am. Chem. Soc. (2009), 131, 4490-4498, “Determining the Effects of Lipophilic Drugs on Membrane Structure by Solid-State NMR Spectroscopy: The Case of the Antioxidant Curcumin.”
  • - Sathiah Thennarasu, Rui Huang, Dong-Kuk Lee, Pei Yang, Lee Maloy, Zhan Chen, and Ayyalusamy Ramamoorthy, Limiting an Antimicrobial Peptide to the Lipid-Water Interface Enhances Its Bacterial Membrane Selectivity: A Case Study of MSI-367, Biochemistry (2010), 49, 10595?10605
  • - Lee, Hee-Jin, Hye-Won Kwon, Jong-Uk Koh, Dong-Kuk Lee, Ja-Young Moon, and Kwang-Hoon Kong, An Efficient Method for the Expression and Reconstitution of Thermostable Mn/Fe Superoxide Dismutase from Aeropyrum pernix K1, J. Microbiol. Biotechnol. (2010), 20(4), 727?731.
  • - Ayyalusamy Ramamoorthy, Dong-Kuk Lee, Tennaru Narasimhaswamy, Ravi P.R. Nanga, Cholesterol reduces pardaxin’s dynamics?a barrel-stavemechanism of membrane disruption investigated by solid-state NMR, Biochimica et Biophysica Acta (BBA), Biomembranes, (2010), 1798, 223-227.

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